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Original Article
- Molecular Investigation of Quinolone Resistance of Quinolone Resistance-Determining Region in
Streptococcus pneumoniae Strains Isolated from Iran Using Polymerase Chain Reaction–Restriction Fragment Length Polymorphism Method - Mohammad Kargar, Fataneh Moein Jahromi, Abbas Doosti, Somayeh Handali
- Osong Public Health Res Perspect. 2014;5(5):245-250. Published online October 31, 2014
- DOI: https://doi.org/10.1016/j.phrp.2014.08.010
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Abstract
PDF - Objectives
The resistance of Streptococcus pneumoniae to the recently available antibiotic treatment has been a growing problem. The aim of the study was to determine the quinolone-resistant strains and detect the presence of mutations in the quinolone resistance-determining regions of the gyrA, parE, and parC genes.
Methods
In this study, for the first time in Iran, the polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) method was used to investigate the presence of mutations at quinolone resistance-determining regions of topoisomerase IV and DNA gyrase on 82 S. pneumoniae strains, among them 45 clinical samples were from patients and 37 from healthy carriers (control group).
Results
In clinical samples, 34 (75.56%) strains contained mutations in the parC gene, 31 (68.89%) carried mutations in the gyrA gene, and 14 (31.11%) had parE gene mutations. Antibiotic susceptibility test was performed using the CLSI (Clinical and Laboratory Standards Institute) criteria on three different generations of quinolone family, with nalidixic acid (82.22%) showing the highest resistance and levofloxacin (42.22%) the least resistance.
Conclusion
Results
indicated that there is a significant correlation between quinolone resistance development and mutations in the parE gene as well as in the parC and gyrA genes. -
Citations
Citations to this article as recorded by
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Muflihah, Ari Hardianto, Pintaka Kusumaningtyas, Sulistyo Prabowo, Yeni Wahyuni Hartati
Heliyon.2023; 9(3): e14418. CrossRef - Mutant selection window of four quinolone antibiotics against clinical isolates of Streptococcus pneumoniae , Haemophilus influenzae and Moraxella catarrhalis
Hajime Nakai, Takumi Sato, Takashi Uno, Emiko Furukawa, Masato Kawamura, Hiroshi Takahashi, Akira Watanabe, Shigeru Fujimura
Journal of Infection and Chemotherapy.2018; 24(2): 83. CrossRef
- DNA-based detection of pork content in food
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